| Allen University | NCAA II | Columbia | SC | Southern Intercollegiate Athletic Conference | https://auyellowjackets.com/sports/softball/ | |
| Anderson University – South Carolina | NCAA II | Anderson | SC | South Atlantic Conference | https://andersonuniversity.edu/softball/ | |
| Benedict College | NCAA II | Columbia | SC | Southern Intercollegiate Athletic Conference | https://benedicttigers.com/sports/softball | |
| Charleston Southern University | NCAA I | Charleston | SC | Big South Conference | https://www.csusports.com/sports/softball | |
| Claflin University | NCAA II | Orangeburg | SC | Central Intercollegiate Athletic Association | https://athletics.claflin.edu/sports/softball | |
| Clemson University | NCAA I | Clemson | SC | Atlantic Coast Conference | https://clemsontigers.com/sports/softball | |
| Coastal Carolina University | NCAA I | Conway | SC | Sun Belt Conference | https://goccusports.com/sports/softball | |
| Coker University | NCAA II | Hartsville | SC | South Atlantic Conference | https://www.cokercobras.com/sports/sball/index | |
| College of Charleston | NCAA I | Charleston | SC | Colonial Athletic Association | https://cofcsports.com/sports/softball | |
| Columbia College – South Carolina | NAIA | Columbia | SC | Appalachian Athletic Conference | https://www.gokoalas.com/sports/sball/index | |
| Converse University | NCAA II | Spartanburg | SC | Conference Carolinas | https://govalkyries.com/sports/softball | |
| Erskine College | NCAA II | Due West | SC | Conference Carolinas | https://erskinesports.com/sports/softball | |
| Florence-Darlington Technical College | JUCO | Florence | SC | REG10N | https://fdtc.prestosports.com/sports/sball/index | |
| Francis Marion University | NCAA II | Florence | SC | Conference Carolinas | https://fmupatriots.com/sports/softball | |
| Furman University | NCAA I | Greenville | SC | Southern Conference | https://furmanpaladins.com/sports/softball | |
| Lander University | NCAA II | Greenwood | SC | Peach Belt Conference | https://landerbearcats.com/sports/softball | |
| Morris College | NCAA II | Sumter | SC | Continental Athletic Conference | https://www.morris.edu/athletics/sports | |
| Newberry College | NCAA II | Newberry | SC | South Atlantic Conference | https://newberrywolves.com/sports/softball | |
| North Greenville University | NCAA II | Tigerville | SC | Conference Carolinas | https://www.nguathletics.com/sports/softball | |
| Presbyterian College | NCAA I | Clinton | SC | Big South Conference | https://gobluehose.com/sports/softball | |
| South Carolina State University | NCAA I | Orangeburg | SC | Mid-Eastern Athletic Conference | https://www.scsuathletics.com/sports/softball | |
| Southern Wesleyan University | NCAA II | Central | SC | Conference Carolinas | https://swuathletics.com/sports/softball | |
| Spartanburg Methodist College | NCAA II | Spartanburg | SC | Continental Athletic Conference | https://www.gosmcpioneers.com/sports/sball/index | |
| University of South Carolina | NCAA I | Columbia | SC | Southeastern Conference | https://gamecocksonline.com/sports/softball/ | |
| University of South Carolina – Beaufort | NCAA II | Bluffton | SC | Peach Belt Conference | https://uscbathletics.com/sports/softball | |
| University of South Carolina – Salkehatchie | JUCO | Walterboro | SC | REG10N | https://www.uscsalkathletics.com/softball | |
| University of South Carolina – Sumter | JUCO | Sumter | SC | REG10N | https://uscfireants.com/sports/sball/index | |
| University of South Carolina – Union | JUCO | Union | SC | REG10N | https://sc.edu/about/system_and_campuses/union/athletics/ | |
| University of South Carolina – Upstate | NCAA I | Spartanburg | SC | Big South Conference | https://upstatespartans.com/sports/softball | |
| University of South Carolina Aiken | NCAA II | Aiken | SC | Peach Belt Conference | https://pacersports.com/sports/softball | |
| Voorhees College | NCAA II | Denmark | SC | HBCU Athletic Conference | https://voorheesathletics.com/sports/softball | |
| Winthrop University | NCAA I | Rock Hill | SC | Big South Conference | https://winthropeagles.com/sports/softball | |
| Wofford College | NCAA I | Spartanburg | SC | Southern Conference | https://woffordterriers.com/sports/softball | |
